These primary linkage maps are the initial step toward the construction of a composite high-density map to facilitate map-based cloning and marker-assisted selection of quantitative trait loci that are essential for the development of comprehensive breeding programs for the guppy. Several RAPD markers were closely linked to or mapped onto the loci for the sex-determining region (SdR), and the sex-linked black caudal-peduncle (Bcp) and red tail (Rdt) genes. Also referred to as RFLP Analysis, the technique takes advantage of the polymorphisms in individual peoples genetic codes. Please enter the biology or biochemistry terms you want to search. Restriction Fragment Length Polymorphism (RFLP) is a molecular method of genetic analysis that allows individuals to be identified based on unique patterns of restriction enzyme cutting in specific regions of DNA. Genome length was estimated at 4410 cMK and 4060 cMK for the respective crosses, with an average physical distance of 166 kbp/cMK. restriction fragment length polymorphism - Terminology of Molecular Biology for restriction fragment length polymorphism GenScript Biology Terms Dictionary This Biology terms dictionary provides query services for biology and biochemistry terms. The Tuxedo ♂♂ × Wildtype ♀♀ and Wildtype ♂♂ × Tuxedo ♀♀ maps spanned 2100 Kosambi centiMorgans (cMK) and 1900 cMK, respectively, in 28 linkage groups. Among the 300 and 276 RAPD markers scored for the respective crosses, linkages were identified for 230 and 212, respectively. Segregation patterns of random amplified polymorphic DNA (RAPD) markers and phenotypic markers were investigated in F2 offspring of Tuxedo ♂♂ × Wildtype ♀♀ and Wildtype ♂♂ × Tuxedo ♀♀ crosses. Genetic linkage maps of the guppy (Poecilia reticulata) were constructed from independent crosses between the Tuxedo strain and a feral line (Wildtype).
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